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American Head & Neck Society
July 21-25, 2012
Metro Toronto Convention Centre
Toronto, ON, Canada


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GENE EXPRESSION PROFILING OF ORAL SQUALORS CELL CARCINOMA BY DIFFERENTIAL DISPLAY RT-PCR AND IDENTIFICATION OF TUMOR BIOMARKERS

Presentation: P159
Topic: Basic Science
Type: Poster
Date: Sunday - Tuesday, July 22 - 24, 2012
Session: Designated Poster viewing times
Authors: Sanjukta Chakraborty, PhD, Nagashri M.N, MD, Azeem Mohiyuddin SM, MD, Arun Kumar, PhD, Gopinath Kodaganur, MD
Institution(s): Indian Institute of Science , HCG Bangalore Institute of Oncology and RL Jalappa Hospital and Research Centre

Oral squamous cell carcinoma (OSCC) is the sixth most common cancer worldwide. Despite progress in therapeutic and surgical treatments, its survival period at 5 years is the lowest among major cancers, and remains unchanged in the last two decades. The growing epidemiological relevance of oral cancer emphasizes the need to better understand the molecular mechanisms underlying this disease and identify predictive tumor markers. To this end, we have used DDRT-PCR to profile the oral tumor transcriptome and identify differentially regulated genes that may function as potential therapeutic targets. Our DDRT-PCR analysis identified 51 differentially expressed fragments of which 25 were revalidated by reverse Northern analysis. Northern blot analysis further corroborated these findings for a few genes. In order to ascertain the utility of some of the identified genes as molecular markers, semi-quantitative RT-PCR analysis was carried out in a panel of matched oral normal and tumor samples, that confirmed GLTP, PCNA, RBM28, C17orf75 and DIAPH1 as significantly unregulated, whereas TNKS2, PAM and TUBB2C showed significant down regulation in tumor samples. Taken together, our DDRT-PCR analysis has revealed several genes, belonging to diverse cellular pathways that have been associated with oral cancer for the first time. Thus, these genes could be investigated as biomarkers and therapeutic targets for OSCC.

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