Archives of Otol
HOME BROWSE TOPICS PRESENTERS SESSIONS

Hosted by
American Head & Neck Society
July 21-25, 2012
Metro Toronto Convention Centre
Toronto, ON, Canada


Tips on Using this Site
Locate an abstract by entering any author's surname into the search box.

Broaden your search by typing only a few letters of a keyword; do not append the wildcard “*” to a search string.

Type too small? In most browsers, press Control “+” to enlarge type size. Reset by pressing Control “0”.

Results. To view an abstract, click on a title within results. Presenters’ names are underlined among the list of authors.
COMPARISON OF HISTOLOGICAL FEATURES SEEN IN PRIMARY OSCC SITES WITH N0, N+ECS-VE AND N+ECS+VE DISEASE AND ORGANOTYPIC MODELS USING PRIMARY EPITHELIAL AND MESENCHYMAL CELL LINES DERIVED FROM THE SAME PATIENTS.

Presentation: P166
Topic: Basic Science
Type: Poster
Date: Sunday - Tuesday, July 22 - 24, 2012
Session: Designated Poster viewing times
Authors: Jagtar Dhanda, MFDS MRCS BScHons, Richard Shaw, FRCS FDS MD, Janet Risk, PhD, Asterios Triantafyllou, MBS PhD, Julia Woolgar, MBBS PhD, Ross Sibson, PhD
Institution(s): University of Liverpool

The hallmarks of cancer have recently been updated to include the additional influences of the tumour microenvironment on the metastastic phenotype. The paracrine interactions of epithelia and the surrounding stromal cells are drivers of aggressive invasive disease associated with metastasis in Oral Squamous Cell Carcinoma (OSCC). Recent evidence has shown that myofibroblast transdifferentiation in surrounding tumour stroma with alpha smooth muscle actin expression (aSMA), and the presence of extracapsular spread (ECS), are the most adverse prognosticators of outcome for OSCC. Our aim was to compare the histological features of primary tumour sites with those seen in three dimensional, organotypic models of invasion. These were developed using primary keratinocyte and fibroblast populations from corresponding patients. Primary tumours associated with N0, N+ECS-ve and N+ECS+ve disease were examined.


Single cell populations were derived from 34 primary oral squamous cell carcinoma (OSCC) sites and classified principally by the pathological node status of the index tumour. Four pN0, one pN+ECS-ve and four pN+ECS+ keratinocyte populations were isolated together with 15 pN0, two pN+ECS- and eight pN+ECS+ fibroblast populations. Organotypic cultures were established using 0.5X106 fibroblasts embedded in a matrigel and type 1 collagen mix (50:50) overlaid with 0.5x105 keratinocytes. After 14 days, the assays were formalin-fixed, paraffin-embedded and H&E stained prior to observations. Collagen gel contraction, an indicator of myofibroblast transdifferentiation, was also measured before and after the addition of keratinocytes.


Features of the primary tumour were compared to the organotypic cultures with description of epithelialisation, invasion (established or microinvasion), pattern of invasion (dyscohesive and non-cohesive) and depth of invasion. Sections were also stained with immunohistochemistry for aSMA and AE1AE3, a pancytokeratin marker, to enable subsequent quantification of invasion using image analysis software. An invasion index was calculated using depth of invasion, area of invasion and number of invading particles.
 

imolope amning graviditetogvit.site imolope orifarm

JAMA Network Logo
© 2023 American Medical Association. All Rights Reserved.      Conditions of Use       Privacy Policy     Toronto skyline by John Vetterli (cc-by-sa-2.0)