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American Head & Neck Society
Annual Meeting, April 10-11, 2013
JW Marriott Grande Lakes
Orlando, Florida

During the
Combined Otolaryngology Spring Meeting
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OCT-4 STEM CELL GENE IS OVEREXPRESSED IN ORAL SQUAMOUS CELL CARCINOMA

Presentation: P006
Topic: Basic Science - Molecular / Cellular Biology
Type: Poster
Date: Wednesday - Thursday, April 10 - 11, 2013
Session: Designated Poster viewing times
Authors: Maria Fernanda D Rodrigues, PhD, Flavia Calo A Xavier, PhD, Eloiza H Tajara, PhD, Head and Neck Genome Project Gencapo, Fabio D Nunes, PhD
Institution(s): Department of Oral Pathology, Dental School University of São Paulo, São Paulo-SP, Brazil

 Oral squamous cell carcinoma (OSCC) contains a heterogeneous population of cancer cells. A rare subpopulation of neoplastic cells, termed cancer stem cells, is capable of initiating, maintaining and expanding the tumor growth. Oct-4 is a surface marker that renders the reprogramming capability of adult cells into germ-line-competent induced pluripotent stem cells. Recently, Oct-4 expression has been shown in human OSCC stem-like cells and related to self-renewal and tumorigenesis via activating its downstream target genes. It was also correlated with worse prognosis of OSCC. To date, few reports described Oct-4 altered expression levels in OSCC. The aim of this study was to analyze Oct-4 gene expression in squamous cell carcinoma of the tongue and floor of the mouth, non-neoplastic margins, OSCC cell lines and normal oral keratinocytes (NOK) obtained from primary culture. Additionally, Oct-4 expression was examined to evaluate the association with the OSCC prognosis. Total RNA from 25 OSCC and 18 non-neoplastic margin tissues and OSCC cel lines (4, 9, 15 and 25) were subjected to quantitative reverse transciptase-polymerase chain reaction (qRT-PCR), with SYBR Green and specific primers for Oct-4 gene. Quantitative analysis was performed according to Pfaffl mathematical model and Oct-4 expression levels was correlated to survival rate. Oct-4 gene revealed higher and statistically significant expression in OSCC samples when compared to margins. Mean expression levels of Oct-4 in OSCC cell lines was comparable to that of NOK. There was no correlation between Oct-4 expression leves and overall survival rate. These results indicate that the abnormal expression of Oct-4  is associated with OSCC.




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