MMPs as Biomarkers for the progression of laryngeal carcinoma

Presentation: P008
Topic: Cancer Biology
Type: Poster
Date:
Session:
Authors: Jyoti Saini, PhD; Jaimanti Bakshi, MS; Atul Kumar, PhD
Institution(s): PGIMER Chandigarh India


Background:

Matrix metalloproteinase (MMPs) belongs to the family of endopeptidase and are known for their properties of degradation of the extracellular matrix. Since MMPs involves in the degradation of the extracellular matrix, they are involved in tumor progression and metastasis. 


Objectives:

We proposed that MMPs could act as biomarkers for the progression of laryngeal carcinoma and therefore, we assessed the expression of MMP-2 and MMP-9 in the tissue sample of patients of laryngeal carcinoma.


Methods:

Total 15 patients of laryngeal carcinoma was recruited in the study from the outpatient department of the institute. The tumor tissue sample was obtained through biopsy after applying the local anesthesia. From the tissue sample, the RNA was isolated and cDNA was prepared using isolated RNA. The RT-PCR was used to study the expression of MMP-2 and MMP-9. Amplified products were quantitated in terms of 2^-ΔCT.


Results:

Both MMP-2 and MMP-9 were showed significantly high expression at advanced stages of cancer compare to the local stages. Also, the expression of MMP-2 was significantly higher compared to the
MMP-9.


Conclusion:

Since MMPs show higher expression at advanced stages of laryngeal carcinoma, they could act as potential biomarkers to assess the progression of laryngeal carcinoma.