Methylation and gene expression differences between ASC/TMS1 and MyD88 genes in healthy and cancer laryngeal tissue

Presentation: P050
Topic: Cancer Biology
Type: Poster
Date:
Session:
Authors: Mario Bilic1; Lana Kovac Bilic1; Jelena Knezevic2; Maja Sutic2
Institution(s): 1University Hospital Center Zagreb, Croatia; 2Ruder Boskovic Institute Zagreb, Croatia


Objective:

There are no biomarkers for diagnosis, prognosis and treatment of patients with laryngeal carcinoma. Methylation changes of ASC/TMS1 and MyD88 genes, in healthy and cancer tissue, might be related with development and progression of cancer. The study explored is there a difference in gene’s methylation in healthy and tumor tissue and does it correlate with protein expression.


Methods:

The total of 36 patients were enrolled in the study. Methylation of bisulphite converted DNA was quantified by pyrosequencing in fresh frozen cancer and adjacent non-malignant tissues.


Results:

The overall methylation of MyD88 gene is significantly higher in healthy tissue and this finding correlates with protein expression and the overall methylation of ASC/TMS1 gene is unchanged but the protein expression of ASC/TMS1 is significantly higher in cancer.


Conclusion:

The methylation status of the ASC/TMS1 and MyD88 genes are promising prognostic biomarker candidates and may lead to earlier detection of laryngeal cancer.